[1]苏作鹏,沈刚,徐福林,等.芝麻素对实验性脑出血后脑损伤的保护作用[J].临床神经外科杂志,2018,15(01):34-37.[doi:10.3969/j.issn.1672-7770.2018.01.008]
 SU Zuo-peng,SHEN Gang,XU Fu-lin,et al.Protection effect of sesamin on brain damage after experimental intracerebral hemorrhage[J].Journal of Clinical Neurosurgery,2018,15(01):34-37.[doi:10.3969/j.issn.1672-7770.2018.01.008]
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芝麻素对实验性脑出血后脑损伤的保护作用()
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《临床神经外科杂志》[ISSN:1672-7770/CN:32-1727/R]

卷:
15
期数:
2018年01期
页码:
34-37
栏目:
论著
出版日期:
2018-02-15

文章信息/Info

Title:
Protection effect of sesamin on brain damage after experimental intracerebral hemorrhage
作者:
苏作鹏沈刚徐福林袁方
201100 上海,上海市闵行区中心医院神经外科(苏作鹏,沈刚,徐福林);上海市第六人民医院神经外科(袁方)
Author(s):
SU Zuo-pengSHEN GangXU Fu-linet al.
Department of Neurosurgery,Central Hospital of Minhang District,Shanghai 201100,China
关键词:
脑出血脑水肿炎症反应芝麻素
Keywords:
sesamincerebral hemorrhagebrain edemablood-brain barrierinflammatory response
分类号:
R743.34
DOI:
10.3969/j.issn.1672-7770.2018.01.008
文献标志码:
A
摘要:
目的 探讨芝麻素对实验性脑出血后继发脑损伤的保护作用。方法 采用自体血脑出血模型,随机将小鼠分为假手术组、脑出血组、脑出血后注射芝麻素组。称量脑组织干湿重来观察脑水肿的变化;通过伊文思蓝渗出量评估血-脑屏障的破坏;同时用免疫荧光双标和Western blot方法检测血-脑屏障结构中紧密连接蛋白的表达改变;采用实时荧光定量PCR检测脑出血周围组织中炎症因子IL-1、IL-6和TNF-α的表达水平。结果 芝麻素显著减轻了脑出血后脑组织的水含量(P<0.05);减少了伊文思蓝的渗出量(P<0.001)和紧密连接蛋白的降解;并降低了炎症因子IL-1β、IL-6和TNF-α的表达水平(均P<0.001)。结论 芝麻素可能是通过抑制炎症反应,改善血-脑屏障的破坏,减轻脑水肿,从而保护脑出血后继发脑损伤。
Abstract:
Objective To explore the protection of sesamin in brain damage after experimental intracerebral hemorrhage.Methods Manufacture of autologous blood cerebral hemorrhage model in mice.Rats were randomly divided into sham operation group,cerebral hemorrhage group and the injection of sesamin in cerebral hemorrhage group.The changes of brain edema by weighing tissue dry wet weight were observed. Blood-brain barrier damage by the evans blue effusion quantity were evaluated.The expression of tight junction protein in the blood brain barrier by double-labelling immunofluorescence and western blot were detected.The expression of inflammation factor IL-1β,IL-6,and TNF-α in cerebral hemorrhage by Real-time fluorescent quantitative PCR were detected.Results Sesamin significantly reduced the severity of brain edema of cerebral hemorrhage(P<0.05).The effusion quantity of evans blue(P<0.001) and the degradation of tight junction protein after cerebral hemorrhage were reduced.The structural integrity of the blood-brain barrier were improved.The inflammatory response after cerebral hemorrhage were suppressed.Conclusion Sesamin probably protect the brain injury after intracerebral hemorrhage by inhibiting the inflammatory reaction,improve the blood brain barrier damage and reduce brain edema.

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更新日期/Last Update: 1900-01-01