[1]叶涵斌,黄维一,任冰焱,等.AHR/SOCS2通路调控氧糖剥夺再灌注星形胶质细胞炎性反应的研究[J].临床神经外科杂志,2019,16(3):235-244.[doi:10.3969/j.issn.1672-7770.2019.03.011]
 YE Han-bin,HUANG Wei-yi,REN Bing-yan,et al.AHR/SOCS2 pathway regulates astrocyte inflammatory response induced by cerebral ischemia reperfusion injury[J].Journal of Clinical Neurosurgery,2019,16(3):235-244.[doi:10.3969/j.issn.1672-7770.2019.03.011]
点击复制

AHR/SOCS2通路调控氧糖剥夺再灌注星形胶质细胞炎性反应的研究 ()
分享到:

《临床神经外科杂志》[ISSN:1672-7770/CN:32-1727/R]

卷:
16
期数:
2019年第3期
页码:
235-244
栏目:
论著
出版日期:
2019-06-15

文章信息/Info

Title:
AHR/SOCS2 pathway regulates astrocyte inflammatory response induced by cerebral ischemia reperfusion injury
作者:
叶涵斌黄维一任冰焱邵君飞
214000 无锡,南京医科大学附属无锡人民医院神经外科(叶涵斌,黄维一,邵君飞),神经内科(任冰焱)
Author(s):
YE Han-bin HUANG Wei-yi REN Bing-yan et al.
Department of Neurosurgery, the Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi 214000, China
关键词:
星形胶质细胞氧糖剥夺再灌注AHRSOCS2炎性因子
Keywords:
astrocyte OGD/R AHR SOCS2 inflammatory factors
分类号:
R739.41
DOI:
10.3969/j.issn.1672-7770.2019.03.011
文献标志码:
A
摘要:
【摘要】目的探讨芳香烃受体(aryl hydrocarbon receptor, AHR)/细胞因子信号转导抑制因子2(suppressor of cytokine signaling 2, SOCS2)通路对氧糖剥夺再灌注(oxygen glucose deprivation/reperfusion,OGD/R)后星形胶质细胞炎性反应的影响。方法体外分离培养乳鼠脑星形胶质细胞,建立OGD/R星形胶质细胞模型。将细胞分为以下各组:ctrl组(正常培养),OGD/R组,OGD/R+阴性对照组,OGD/R+AHR过表达组,OGD/R+AHR过表达+SOCS2敲减组。采用Western blot和RT-PCR检测各组AHR、SOCS2和炎性因子表达,验证SOCS2能否阻断AHR调控的OGD/R后星形胶质细胞炎性因子肿瘤坏死因子(tumor necrosis factor α,TNF-α)、白介素6 (interleukin 6, IL-6)、诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)的表达。结果AHR、SOCS2过表达后TNF-α、IL-6、iNOS表达水平升高,抑制SOCS2表达可阻断AHR对TNF-α、IL-6、iNOS表达的上调作用。结论AHR/SOCS2通路参与调控OGD/R后的星形胶质细胞炎性反应。
Abstract:
Abstract: ObjectiveTo investigate the role of AHR/SOCS2 pathway on astrocyte inflammatory response after oxygen deprivation. MethodsAstrocytes (AS) were isolated and cultured in vitro to establish astrocyte oxygen glucose deprivation(OGD/R) model. AS were divided into the following groups as ctrl group(normal culture), OGD/R group, OGD/R+negative control group, OGD/R+AHR overexpression group, OGD/R+AHR overexpression+SOCS2 knockdown group. Western blot and RT-PCR were used to detect the expression of AHR, SOCS2 and inflammatory factors in each group. It was used to confirm whether SOCS2 could block the expression of AS inflammatory factors TNF-α, IL-6 and iNOS after OGD/R which was regulated by AHR. ResultsThe expression levels of TNF-α, IL-6 and iNOS were increased after overexpression of AHR and SOCS2. Knocking down SOCS2 could block the up-regulation of TNF-α, IL-6 and iNOS which was caused by AHR. ConclusionThe AHR/SOCS2 pathway is involved in the regulation of AS inflammation after OGD/R.

相似文献/References:

[1]钱腾达,张斌,刘彦,等.再程序化星形胶质细胞的制备及体外定向分化为神经元的研究[J].临床神经外科杂志,2015,(04):256.
[2]赵学渊,蔡青,冯达云,等.组蛋白去乙酰化酶2抑制剂在蛛网膜下腔出血模型动物认知障碍调节中的作用[J].临床神经外科杂志,2019,16(5):410.[doi:10.3969/j.issn.1672-7770.2019.05.009]
 ZHAO Xue-yuan,CAI Qing,FENG Da-yun,et al.Role of HDAC2i in regulation of cognitive impairment in animal models of subarachnoid hemorrhage[J].Journal of Clinical Neurosurgery,2019,16(3):410.[doi:10.3969/j.issn.1672-7770.2019.05.009]

更新日期/Last Update: 2019-06-15