[1]丁浩,周涛,成宜军,等.下调磷酸甘油酸激酶1增强胶质瘤U251细胞的放射敏感性[J].临床神经外科杂志,2014,(05):321-325.
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下调磷酸甘油酸激酶1增强胶质瘤U251细胞的放射敏感性()
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《临床神经外科杂志》[ISSN:1672-7770/CN:32-1727/R]

卷:
期数:
2014年05期
页码:
321-325
栏目:
胶质瘤基础研究
出版日期:
2014-10-31

文章信息/Info

Title:
Down-regulation of Phosphoglycerate kinase 1 enhances the radiosensitivity of glioma U251 cells
作者:
丁浩 周涛 成宜军 阎华 张锐 肖红 刘宏毅
南京医科大学附属脑科医院
关键词:
磷酸甘油酸激酶1放射敏感性胶质瘤U251细胞
Keywords:
Phosphoglycerate kinase 1 Radiosensitivity Glioma U251 cells
分类号:
R739.41
文献标志码:
A
摘要:
目的 研究磷酸甘油酸激酶1(PGK1)对胶质瘤U251细胞放射敏感性的影响。方法 建立放射抵抗的U251(RR-U251)细胞;LipofectamineTM2000方法将抑制PGK1表达的shRNA-PGK1质粒或对照shRNA-NC转染至U251细胞;荧光定量PCR及Western Blot检测PGK1的表达;MTT检测细胞相对存活率,划痕实验检测细胞迁移能力,Transwell小室实验检测细胞侵袭能力。结果 与正常U251细胞相比,RR-U251细胞中PGK1的表达明显增高;放射处理后,RR-U251细胞的相对存活率升高,迁移能力及侵袭能力增强。转染shRNA-PGK1的细胞接受放射处理后,与对照组相比,细胞的相对存活率降低,迁移能力以及侵袭能力减弱。结论 下调U251细胞中PGK1的表达可增加其放射敏感性,提示PGK1可能成为增强放射敏感性的调控靶点。
Abstract:
Objective This study was designed to explore the role of Phosphoglycerate kinase 1 (PGK1) in the radiosensitivity of glioma U251 cells. Methods Radioresistant U251 cells (RR-U251) were established. The shRNA for PGK1 and the control shRNA were transfected to U251 cells using lipofectamineTM2000, and real-time quantitative fluorescent PCR and western blot analysis were performed to evaluate PGK1 expression. The cell viability was determined by MTT assay. The wound-healing assay (WHA) method was used to evaluate cell migration ability. Cell invasion abilities were examined using Transwell culture chamber system. Results Our results showed that the expression of PGK1 was significantly increased in RR-U251 cells as compared to normal U251 cells. After irradiation, the cell viability was significantly higher in RR-U251 cells compared with normal U251 cells, and migration ability and invasion ability was also enhanced. Down-regulating PGK1 using shRNA could induce a significantly down-regulated cell viability, and decreased the ability of migration and invasion. Conclusion These findings suggest that down-regulation of PGK1 could enhance radiosensitivity in human U251 cells, and PGK1 may serves as a target for enhancing radiosensitivity.

相似文献/References:

[1]丁浩,刘宏毅.脑胶质瘤放射敏感性相关分子机制的研究进展[J].临床神经外科杂志,2014,(03):237.
[2]刘宇驰,赵梦洁,孙文博,等.下调磷酸甘油酸激酶1对胶质瘤U373细胞的放疗增敏作用及其机制的研究[J].临床神经外科杂志,2019,16(2):153.[doi:10.3969/j.issn.1672-7770.2019.02.012]
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[4]王冉冉,杜华情,杨坤,等.原肌球蛋白1与胶质瘤放射敏感性的体外研究[J].临床神经外科杂志,2020,17(03):311.[doi:DOI:10.3969/j.issn.1672-7770.2020.03.015]
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备注/Memo

备注/Memo:
[收稿日期] 2014-02-13 [基金项目] 国家自然科学基金
更新日期/Last Update: 2014-10-15