[1]廖克曼,季卫阳,鲁晓杰.miR-150*修饰骨髓间充质干细胞来源的exosome对胶质瘤细胞的影响[J].临床神经外科杂志,2015,(04):274-278.
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miR-150*修饰骨髓间充质干细胞来源的exosome对胶质瘤细胞的影响()
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《临床神经外科杂志》[ISSN:1672-7770/CN:32-1727/R]

卷:
期数:
2015年04期
页码:
274-278
栏目:
论著
出版日期:
2015-08-15

文章信息/Info

Title:
miR150*-Loaded exosomes from Marrow Stromal Cells Could Inhibit Glioma Cells Growth
作者:
廖克曼 季卫阳 鲁晓杰
南京医科大学附属无锡第二医院
关键词:
miR-150*胶质瘤exosome增殖凋亡
Keywords:
miR-150* gliomaexosomeproliferationapoptosis
分类号:
R739.41
文献标志码:
A
摘要:
目的 研究miR-150*修饰对骨髓间充质干细胞来源的 exosome对胶质瘤细胞的影响 方法 qRT-PCR检测miR-150*在胶质母细胞瘤组织与正常组织间的表达量差异。培养骨髓间充质干细胞(Bone mesenchymal stem cells,BMSCs),分别转染miR-150* 模拟物和阴性对照序列,上调BMSCs中miR-150*表达水平,提取BMSCs培养基中的exosome。Western blot验证exosomal的表面标记蛋白CD63和flotillin-1,电镜下观察exosome的形态。CCK-8和细胞周期实验验证miR-150*修饰BMSCs来源的exosome对胶质瘤细胞的影响。结果 miR-150*在胶质母细胞瘤组织中表达明显低于正常脑组织,转染miR-150* 模拟物能明显提高BMSCs来源的exosome中miR-150*的表达。miR-150*修饰BMSCs来源的exosome能有效抑制胶质瘤细胞的增殖,促进细胞凋亡。 结论 miR-150*在胶质母细胞瘤组织中低表达,miR-150*修饰BMSCs来源的exosome对胶质瘤细胞有抗增殖,促凋亡的作用。因此exosome可作为一种有效的基因治疗载体。
Abstract:
Objective MiRNA-based therapeutics hold great promise for tumor suppression, this study was to investigate the effect of miR150*-loaded exosomes on regulation of glioma cells proliferation and cell cycle. Methods Quantitative real-time PCR on 15 glioblastoma tissues samples and normal controls were used to confirm the miR-150* expression level. Western blotting analysis and electron microscopy were employed to test exosomal biomarkers and their morphology. Transfection assay were used to collect miR150*-loaded exosomes from bone marrow mesenchymal stem cells (BMSCs) culture medium. CCK-8 and cell cycle assays were used to analyze miR150*-loaded exosomes effects on glioma cells.Results: Level of miR150* expression was much lower in glioblastoma than in normal tissues. Transfection assay successfully acquired miR150*-loaded exosomes which derived from bone marrow mesenchymal stem cells (BMSCs). Furthermore, miR150*-loaded exosomes could largely inhibited glioma cells proliferation and suppress cell cycle progression. Cell counting kit 8 (CCK-8) assays also demonstrated miR150* delivered in exosomes was much less toxic. Conclusions: this study demonstrated miR150* is down-regulated in glioblastoma. MiR150*-loaded exosomes could suppress glioma cells and exosomes may be a potentially efficient therapeutic delivery system.

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备注/Memo

备注/Memo:
[收稿日期] 2015-03-16 [基金项目]无锡市医管中心重大项目
更新日期/Last Update: 2015-08-15