[1]王瑞彤,闫昕,陈辉,等.miR-155在胶质瘤干细胞样细胞的表达及临床意义[J].临床神经外科杂志,2019,16(4):277-281.[doi:10.3969/j.issn.1672-7770.2019.04.001]
 WANG Rui-tong,YAN Xin,CHEN Hui,et al.The expression of miR-155 in GSCs and its clinical significance[J].Journal of Clinical Neurosurgery,2019,16(4):277-281.[doi:10.3969/j.issn.1672-7770.2019.04.001]
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miR-155在胶质瘤干细胞样细胞的表达及临床意义()
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《临床神经外科杂志》[ISSN:1672-7770/CN:32-1727/R]

卷:
16
期数:
2019年第4期
页码:
277-281
栏目:
胶质瘤基础研究专题
出版日期:
2019-08-15

文章信息/Info

Title:
The expression of miR-155 in GSCs and its clinical significance
作者:
王瑞彤闫昕陈辉赵明明王淑为刘爽尹丰
100039 北京,航天中心医院神经内科(王瑞彤),神经外科(闫昕,陈辉,尹丰);海军总医院神经外科(赵明明,王淑为,刘爽)
Author(s):
WANG Rui-tong YAN Xin CHEN Hui et al.
Department of Neurology, Aerospace Center Hospital, Beijing 100039, China
关键词:
miR-155胶质瘤胶质瘤干细胞样细胞神经干细胞预后
Keywords:
miR-155 glioblastoma glioblastoma stem-like cells neural stem cells prognosis of glioma
分类号:
R739.41
DOI:
10.3969/j.issn.1672-7770.2019.04.001
文献标志码:
A
摘要:
【摘要】目的研究miR-155在人胶质瘤干细胞样细胞(glioblastoma stem-like cells,GSCs)与正常神经干细胞(neural stem cells,NSCs),及不同病理级别脑胶质瘤组织的表达差异,其表达水平与胶质瘤恶性程度及预后的关系。方法采用神经球(neurosphere)培养法体外分离扩增人GSCs与NSCs,并进行免疫荧光及移植瘤实验鉴定。用qRT-PCR的方法检测miR-155在GSCs、NSCs及不同级别胶质瘤和正常脑组织的表达水平。结合患者的预后,进行Kaplan-Meier生存曲线分析。结果GSCs的miR-155表达水平明显高于NSCs(P<0.05);随着胶质瘤级别的增高,miR-155的表达水平也明显增高,WHO Ⅲ级、Ⅳ级恶性胶质瘤的miR-155表达水平明显高于正常脑组织(均P<0.05)。miR-155高表达与恶性胶质瘤的不良预后密切相关。结论miR-155在GSCs及高级别胶质瘤组织中的表达水平增高,其表达水平可作为判定胶质瘤预后的标志物。
Abstract:
Abstract: ObjectiveTo study the different expression of miR-155 in glioblastoma stem-like cells(GSCs), neural stem cells(NSCs) and different grades glioma. To clarify the relationship of miR-155 expression, degree of malignancy and prognosis of glioma patients. MethodsGSCs and NSCs were isolated in vitro using neurosphere culture, then characteristics of the GSCs and NSCs were identified by experiments of immunoflurescence and xenograft. QRT PCR technology was used to detect the expression levels of miR-155 in GSCs, NSCs, gliomas of different grades and normal brain tissues. Kaplan-meier survival curve analysis was performed in combination with patient prognosis. ResultsThe expression of miR-155 increased significantly in GSCs compared with NSCs(P<0.05), and also increased with the malignant degree of glioma.The expression level of miR-155 in malignant glioma(WHO Ⅲ, Ⅳ) was obviously higher than that of normal brain tissue(all P<0.05). The high expression of miR-155 was closely related to the poor prognosis of glioma. ConclusionThe expression of miR-155 elevates in GSCs and malignant glioma specimens, and can be considered as biomarker for diagnosis and prognosis judgment of glioma.

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更新日期/Last Update: 2019-08-01