[1]郭中叶,蒋世一,邱云,等.RNA结合蛋白RBM38表达对胶质瘤细胞增殖、迁移、侵袭能力的影响[J].临床神经外科杂志,2019,16(4):282-288.[doi:10.3969/j.issn.1672-7770.2019.04.002]
 GUO Zhong-ye,JIANG Shi-yi,QIU Yun,et al.Effect of RNA-binding motif protein 38 on proliferation, migration and invasion of glioma cells[J].Journal of Clinical Neurosurgery,2019,16(4):282-288.[doi:10.3969/j.issn.1672-7770.2019.04.002]
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RNA结合蛋白RBM38表达对胶质瘤细胞增殖、迁移、侵袭能力的影响()
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《临床神经外科杂志》[ISSN:1672-7770/CN:32-1727/R]

卷:
16
期数:
2019年第4期
页码:
282-288
栏目:
胶质瘤基础研究专题
出版日期:
2019-08-15

文章信息/Info

Title:
Effect of RNA-binding motif protein 38 on proliferation, migration and invasion of glioma cells
作者:
郭中叶蒋世一邱云廖克曼侯鹏鲁晓杰
214000 无锡,南京医科大学附属无锡第二医院神经外科(郭中叶,邱云,候鹏,鲁晓杰);宜兴市第一人民医院神经外科(蒋世一);上海交通大学附属仁济医院神经外科(廖克曼)
Author(s):
GUO Zhong-ye JIANG Shi-yi QIU Yun et al.
Department of Neurosurgery, The Affiliated Wuxi No.2 People’s Hospital of Nanjing Medical University, Wuxi 214000, China
关键词:
RNA结合蛋白RNA结合基序蛋白38胶质瘤细胞增殖细胞迁移
Keywords:
RNA-binding protein RNA-binding motif protein 38 glioma cell proliferation cell migration
分类号:
R739.41
DOI:
10.3969/j.issn.1672-7770.2019.04.002
文献标志码:
A
摘要:
【摘要】目的探讨RNA结合蛋白RBM38(RNA结合基序蛋白38,RNA-binding motif protein 38)在胶质瘤组织和细胞中的表达水平及其对胶质瘤细胞增殖、迁移、侵袭和凋亡的调节作用。方法采用实时荧光定量PCR(quantitative real-time PCR,qRT-PCR)检测胶质瘤组织和瘤旁正常组织的RBM38表达水平。采用qRT-PCR和Western blot检测胶质瘤细胞U87、U251和正常星形胶质细胞HA1800的RBM38表达水平。用免疫组化染色检测59例胶质瘤组织的RBM38表达水平,并分析其与临床病理资料的关系。将靶向RBM38的特异性小干扰RNA运用脂质体介导转染法转染至胶质瘤细胞U87和U251。应用CCK-8(cholecystokinin-8)增殖实验、划痕实验、Transwell小室实验、流式细胞仪测凋亡细胞,检测RBM38对于胶质瘤细胞增殖、迁移、侵袭和凋亡能力的影响。采用qRT-PCR检测抑制RBM38表达后肿瘤细胞P53基因的表达水平。结果相较于瘤旁的脑组织,胶质瘤组织RBM38的表达量明显升高(P<0.05)。胶质瘤细胞中的RBM38表达水平明显高于正常胶质细胞(均P<0.001)。免疫组化结果显示,与低级别胶质瘤相比,高级别胶质瘤的RBM38表达水平更高(P<0.05)。抑制RBM38的表达后,胶质瘤细胞U87和U251的增殖速率明显下降、迁移距离变短、能透过生物膜的细胞数目明显下降(均P<0.05)。下调了RBM38的表达后胶质瘤细胞的凋亡比率升高(P<0.05)。被抑制了RBM38表达的胶质瘤细胞P53基因的表达水平升高(P<0.05)。结论RBM38在胶质瘤组织及细胞系中呈高表达,RBM38的高表达与胶质瘤的临床分级密切相关。下调RBM38的表达后能抑制胶质瘤细胞的增殖、迁移、侵袭能力,促进细胞的凋亡,并使P53基因的表达水平增高;可能为临床诊断和治疗胶质瘤提供新的靶点。
Abstract:
Abstract: ObjectiveTo investigate the expression level of RNA-binding motif protein 38(RBM38) in glioma tissues and cells,and to identify its effects on the proliferation, migration, invasion and apoptosis of glioma cells. MethodsRBM38 mRNA levels in glioma tissues and normal adjacent tissues were analyzed using qRT-PCR(quantitative real-time PCR). RBM38 levels in glioblastoma cell line U87, U251and human astrocyte HA1800 cells were detected using qRT-PCR and Western Blot. Additionally, the correlation between RBM38 expression in glioma tissues and the corresponding clinical pathological characteristics of the patients was explored, based on the IHC results of RMB38 expression in 59 human glioma tissues. Specific small interfering RNAs targeting RBM38 were transfected into glioma cell lines U87 and U251 using liposome-mediated transfection. The effects of RBM38 on the proliferation, migration, invasion and apoptosis of glioma cells were examined by CCK-8 proliferation assay, scratch assay, transwell chamber assay and flow cytometry assay respectively. The qRT-PCR was conducted to detect the P53 expression in the RBM38-depleted glioma cells. ResultsThe expression of RBM38 in glioma tissues was significantly higher, in comparison with that of normal adjacent tissues(P<0.05). RBM38 levels were significantly up-regulated in glioma cell, compared with those in normal cells(all P<0.001). Immunohistochemical results indicated RBM38 expression was higher in high-grade gliomas compared with low-grade gliomas(P<0.05). The inhibition of RBM38 expression resulted in significantly reduced proliferation rate of U87 and U251 cells(P<0.05), suppressed migration and invasion capacity of glioma cells(P<0.05), and significantly enhanced apoptosis rate of glioma cells(P<0.05). The expression level of P53 gene was significantly increased in the RBM38-depleted glioma cells(P<0.05). ConclusionsRBM38 expression is remarkably high in glioma tissues and cell lines. The high expression level of RBM38 is closely related to the clinical grade of glioma. Down-regulation of RBM38 can inhibit the proliferation, migration, invasion of glioma cells, promote cellular apoptosis, and improve the expression of P53 gene. The data demonstrate that RBM38 can be used as novel target for the clinical diagnosis and intervention of glioma.
更新日期/Last Update: 2019-08-01