[1]杨晶晶,王大维,陈一元,等.NR2C2基因在泌乳素瘤的表达及其意义[J].临床神经外科杂志,2019,16(5):425-429.[doi:10.3969/j.issn.1672-7770.2019.05.012]
 YANG Jing-jing,WANG Da-wei,CHEN Yi-yuan,et al.Expression and significance of NR2C2 gene in prolactinoma[J].Journal of Clinical Neurosurgery,2019,16(5):425-429.[doi:10.3969/j.issn.1672-7770.2019.05.012]
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NR2C2基因在泌乳素瘤的表达及其意义()
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《临床神经外科杂志》[ISSN:1672-7770/CN:32-1727/R]

卷:
16
期数:
2019年第5期
页码:
425-429
栏目:
论著
出版日期:
2019-10-15

文章信息/Info

Title:
Expression and significance of NR2C2 gene in prolactinoma
作者:
杨晶晶王大维陈一元刘潜张亚卓高华
100070 北京,北京市神经外科研究所中心实验室(杨晶晶),细胞生物室(陈一元,刘潜,张亚卓,高华);首都医科大学基础医学院(王大维)
Author(s):
YANG Jing-jing WANG Da-wei CHEN Yi-yuan et al.
Department of Cell Biology, Beijing Neurosurgical Institute, Capital Medical University, Beijing 100070, China
关键词:
泌乳素瘤核受体亚家族2c成员2凋亡细胞增殖
Keywords:
prolactinomas nuclear receptor subfamily 2 group C member 2 apoptosis cell proliferation
分类号:
R736.4
DOI:
10.3969/j.issn.1672-7770.2019.05.012
文献标志码:
A
摘要:
【摘要】目的 探讨核受体亚家族2c成员2(nuclear receptor subfamily 2 group C member 2,NR2C2)在泌乳素瘤的表达水平及其与临床特征的关系。方法采用免疫组化染色和蛋白印迹实验检测46例泌乳素瘤患者的肿瘤组织NR2C2表达水平。用RNA干扰技术敲低MMQ细胞NR2C2表达水平,行细胞增殖实验检测细胞活力,流式细胞仪检测细胞凋亡率。结果按照Knosp分级将患者分为侵袭组和非侵袭组。侵袭组NR2C2表达的免疫组化评分为148.2±42.7,非侵袭组为45.2±28.72;NR2C2表达水平与肿瘤大小成正相关(r=0.327,P=0.039);疏松颗粒型患者的表达水平更高(P=0.003)。蛋白印迹实验检测NR2C2蛋白水平,筛选出Sh-B(23.4%)和Sh-C(34%)片段;干扰24 h、48 h和72 h后,与对照组相比Sh-B组的细胞活力分别下降24.8%、39.2%和48.3%,Sh-C组分别下降19.1%、26.4%和32.5%(均P<0.05)。干扰24 h后,Sh-B组Annexin V阳性细胞为(10.3±3.1)%,PI阳性细胞为(7.3±2.1)%;Sh-C组分别为(8.5±2.3)%和(5.6±1.8)%;均明显高于对照组(均P<0.05)。结论NR2C2能促进肿瘤生长,与催乳素腺瘤的发生发展密切相关。
Abstract:
Abstract: ObjectiveTo testify the expression of nuclear receptor subfamily 2 group C member 2 (NR2C2) in the prolactinomas, and analyze the relationship between NR2C2 and clinical features. MethodsImmunohistochemical staining and Western blotting were used to detect the expression of NR2C2 in 46 prolactinoma patients. The relationship between NR2C2 and clinical features was analyzed by follow-up data. RNA interference technique(RNAi) knocked down the NR2C2 level in MMQ cells, and cell viability was detected by cell proliferation assay. Flow cytometry was used to detect the apoptosis level by Annexin V/PI staining. ResultsThe patients were divided into invasive group and non-invasive group according to Knosp classification. The H-score of NR2C2 in the invasive group was 148.2±42.7, and 45.2±28.72 in the non-invasive group(P<0.01). The level of NR2C2 was positively correlated with tumor size(r=0.327,P=0.039), and there was higher NR2C2 level in patients with loose granules than those in dense granules(P=0.003). Western blot experiments filtered the fragments Sh-B(23.4%) and Sh-C(34%) compared to NR2C2 protein level in control MMQ cell(P<0.01). The cell viability of Sh-B group reduced 24.8%, 39.2% and 48.3%, and Sh-C group was 19.1%, 26.4% and 32.5% compared to control cell after 24 h, 48 h and 72 h transfection(all P<0.05). After 24 h RNAi, Annexin V positive cells were (10.3±3.1)% in the Sh-B group and(8.5±2.3)% in Sh-C group, and PI positive cells were(7.3±2.1)% and (5.6±1.8)% separately(all P<0.05). ConclusionNR2C2 is closely related to the tumorigenesis and development of prolactin adenoma.
更新日期/Last Update: 2019-10-15