[1]许强华,刘甜,陈新军,等.TSA联合HSV-Ⅰ型对C6细胞增殖、凋亡的影响[J].临床神经外科杂志,2019,16(6):472-475.[doi:10.3969/j.issn.1672-7770.2019.06.003]
 XU Qiang-hua,LIU Tian,CHEN Xin-jun,et al.Effects of TSA combined with HSV-1 on proliferation,apoptosis in C6 cells[J].Journal of Clinical Neurosurgery,2019,16(6):472-475.[doi:10.3969/j.issn.1672-7770.2019.06.003]
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TSA联合HSV-Ⅰ型对C6细胞增殖、凋亡的影响()
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《临床神经外科杂志》[ISSN:1672-7770/CN:32-1727/R]

卷:
16
期数:
2019年第6期
页码:
472-475
栏目:
胶质瘤研究
出版日期:
2019-12-10

文章信息/Info

Title:
Effects of TSA combined with HSV-1 on proliferation,apoptosis in C6 cells
作者:
许强华刘甜陈新军陈治军刘平非谢腾陈晓巍
448000 荆门,湖北省荆门市第一人民医院神经外科(许强华,陈治军,刘平非,谢腾,陈晓巍),眼科(刘甜);武汉大学中南医院神经外科(陈新军)
Author(s):
XU Qiang-hua LIU Tian CHEN Xin-jun et al.
Department of Neurosurgery, The First People’s Hospital of Jingmen, Jingmen 448000, China
关键词:
曲古抑菌素A单纯疱疹病毒Ⅰ型胶质瘤增殖凋亡
Keywords:
TSA type Ⅰ herpes simplex virus glioma proliferation apoptosis
分类号:
Q2-33
DOI:
10.3969/j.issn.1672-7770.2019.06.003
文献标志码:
A
摘要:
【摘要】目的 研究曲古抑菌素A(TSA)联合单纯疱疹病毒Ⅰ型(HSV-1)对肿瘤细胞增殖、凋亡的影响。方法以0.5×10-3 μmol/L TSA和10MOI HSV-1及0.5×10-3 μmol/L TSA与10MOI HSV-1联合作用于C6鼠胶质瘤细胞;分别于48 h、72 h后,用CKK-8法检测各组细胞的增殖活性,流式细胞仪检测各组细胞凋亡率。结果48 h后,TSA+HSV-1组C6细胞较单一TSA组、单一HSV-1组C6细胞的增殖活性率明显降低(P<0.05,P<0.01);72 h后,TSA+HSV-1联合组C6细胞较单一TSA组和单一HSV-1组C6细胞的增殖活性率明显降低(均P<0.01)。48 h、72 h后,TSA+HSV-1组C6细胞较单一TSA组和单一HSV-1组C6细胞的凋亡率明显升高(均P<0.01)。结论TSA联合HSV-1对体外培养的C6细胞具有协同或叠加杀伤作用。
Abstract:
Abstract: ObjectiveC6 cells are cultured in vitro as models to observe kojic ancient bacteriostatic A(TSA) combine type Ⅰ herpes simplex virus(HSV-1) effects on tumor cell proliferation and apoptosis. MethodsC6 cells in vitro were treated separately with 0.5×10-3 μmol/L TSA, 10MOI HSV-1 and 0.5×10-3 μmol/L TSA combined with 10MOI HSV-1 for 48 h and 72 h. The CCK-8 assay was used to observe the proliferation of C6 cells. The rate of apoptosis was detected by Flow Cytometry. ResultsThe proliferation activity rate of TSA+hsv-1 group was lower than that of TSA group and HSV-1 group 48 h later(P<0.05, P<0.01). After 72 h, the proliferation activity rate of C6 cells in the TSA+ HSV-1 combined group was significantly lower than that in the TSA group and the HSV-1 group(all P<0.01). At 48 h and 72 h, the apoptosis rate of C6 cells in TSA+HSV-1 group was significantly higher than that in TSA group and HSV group(all P<0.01). ConclusionTSA combined with HSV-1 has synergistic or superimposed killing effect on C6 cells cultured in vitro.
更新日期/Last Update: 2019-12-10