[1]周培杰,钱中润,刘伟,等.Eya3通过Hippo信号通路调控胶质瘤细胞化疗耐药的研究[J].临床神经外科杂志,2019,16(06):481-486.[doi:10.3969/j.issn.1672-7770.2019.06.005]
 ZHOU Pei-jie,QIAN Zhong-run,LIU Wei,et al.Studies on role of Eya3 on chemoresistance of glioma cells by regulating Hippo signaling pathway[J].Journal of Clinical Neurosurgery,2019,16(06):481-486.[doi:10.3969/j.issn.1672-7770.2019.06.005]
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Eya3通过Hippo信号通路调控胶质瘤细胞化疗耐药的研究()
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《临床神经外科杂志》[ISSN:1672-7770/CN:32-1727/R]

卷:
16
期数:
2019年06期
页码:
481-486
栏目:
专家共识
出版日期:
2019-12-03

文章信息/Info

Title:
Studies on role of Eya3 on chemoresistance of glioma cells by regulating Hippo signaling pathway
作者:
周培杰钱中润刘伟罗文广张红雁
230001 合肥,中国科学技术大学附属第一医院(安徽省立医院)肿瘤放疗科(周培杰,刘伟,罗文广,张红雁),神经外科(钱中润)
Author(s):
ZHOU Pei-jie QIAN Zhong-run LIU Wei et al.
Department of Tumor Radiotherapy, The First Affiliated Hospital of USTC, Division of Life
关键词:
眼缺失同源物3干性维持替莫唑胺耐药Hippo通路胶质瘤
Keywords:
Eya3 stemness temozolomide-resistance Hippo pathway glioblastoma
分类号:
R739.41
DOI:
10.3969/j.issn.1672-7770.2019.06.005
文献标志码:
A
摘要:
【摘要】目的探讨眼缺失同源物3(eyes absent homolog 3,Eya3)蛋白是否通过调控Hippo通路进而影响胶质瘤细胞的化疗耐药。方法应用免疫组化及Western blotting检测Eya3在胶质瘤组织及细胞中的表达水平;MTT实验检测过表达或沉默Eya3对U87细胞化疗耐药性的影响;3D成球及迁移/侵袭实验分别检测过表达或沉默Eya3对U87细胞干性及迁移/侵袭能力的影响。采用Western blotting实验检测Eya3对Hippo通路磷酸化的影响;染色质免疫共沉淀实验检测Sox2和Slug基因转录水平。结果Eya3在胶质瘤组织和细胞中高表达。过表达Eya3的U87细胞耐药性、干性及迁移/侵袭能力显著增强,沉默Eya3则反之。Eya3抑制Hippo通路磷酸化并促进YAP入核,入核增加的YAP增强Sox2和Slug基因转录。结论Eya3通过调控Hippo通路活性影响胶质瘤细胞的化疗耐药。
Abstract:
Abstract: ObjectiveTo explore whether Eya3 protein(Eyes absent homolog 3) affects the chemoresistance of glioma cells by regulating the Hippo pathway. MethodsImmunohistochemistry and Western blotting were used to detect the expression of Eya3 in glioma tissues and cell lines. The effect of overexpression or silencing of Eya3 on chemotherapy resistance of U87 cells was detected by MTT assay. The effects of over-expression or silencing of Eya3 on the stemness and migration/invasion ability of U87 cells were examined by three-dimensional balling and migration/invasion experiments, respectively. The effect of Eya3 on the phosphorylation of Hippo pathway was detected by Western blotting. The transcription levels of Sox 2 and Slug genes were detected by ChIP(chromatin immunoprecipitation assay) assay. ResultsEya3 was highly expressed in glioma tissues and cells. After overexpression of Eya3, the resistance, stemness, migration and invasion ability of U87 cells increased significantly, while silencing of Eya3 was the opposite. Eya3 inhibits the phosphorylation of Hippo pathway and promotes the nuclear translocation of YAP. The increased YAP enhances the transcription of Sox2 and Slug genes. ConclusionEya3 affects chemoresistance of glioma cells by regulating the activity of Hippo pathway.
更新日期/Last Update: 2019-12-10