[1]肖广庆,刘希光,伦志刚,等.Prdx6对神经干细胞增殖和分化的影响研究[J].临床神经外科杂志,2020,17(01):61-65.[doi:DOI:10.3969/j.issn.1672-7770.2020.01.014]
 XIAO Guang-qing,LIU Xi-guang,LUN Zhi-gang,et al.Effect of Prdx6 on proliferation and differentiation of neural stem cells[J].Journal of Clinical Neurosurgery,2020,17(01):61-65.[doi:DOI:10.3969/j.issn.1672-7770.2020.01.014]
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Prdx6对神经干细胞增殖和分化的影响研究()
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《临床神经外科杂志》[ISSN:1672-7770/CN:32-1727/R]

卷:
17
期数:
2020年01期
页码:
61-65
栏目:
论著
出版日期:
2020-02-15

文章信息/Info

Title:
Effect of Prdx6 on proliferation and differentiation of neural stem cells
文章编号:
202001014
作者:
肖广庆刘希光伦志刚陈慧珍李爱民
221004 徐州,徐州医科大学研究生学院(肖广庆,伦志刚);徐州医科大学附属连云港医院神经外科(刘希光,陈慧珍,李爱民)
Author(s):
XIAO Guang-qing LIU Xi-guang LUN Zhi-gang et al.
Graduate School of Xuzhou Medical University, Xuzhou 221004, China
关键词:
Prdx6神经干细胞增殖分化
Keywords:
Prdx6 neural stem cells proliferation differentiation
分类号:
RQ254;Q421
DOI:
DOI:10.3969/j.issn.1672-7770.2020.01.014
文献标志码:
A
摘要:
目的探讨过氧化物氧化还原酶6(Prdx6)对神经干细胞的增殖和分化的影响。方法(1)采用悬浮培养法从新生SD大鼠脑分离培养神经干细胞,传至第三代后进行神经干细胞特异性标记蛋白巢蛋白(Nestin)的免疫荧光检测;(2)细胞实验分组:溶媒组、Prdx6 1 ng/mL、10 ng/mL、100 ng/mL、1 μg/mL组;(3)采用台盼蓝细胞计数法计数各组细胞培养第1 d、第2 d、第3 d、第4 d和第5 d的细胞数,并绘制细胞生长曲线;(4)细胞增殖实验观测各组细胞形成神经球的直径,用Cell Counting kit-8(CCK-8)试剂盒检测各组细胞在450 nm处的吸光度值;(5)在细胞分化实验中,通过免疫荧光染色检测神经干细胞分化后神经元特异性烯醇化酶(neuron specific enolase,NSE)、胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)阳性细胞数。结果(1)体外培养得到悬浮生长的神经干细胞,免疫荧光染色Nestin呈阳性;(2)Prdx6 100 ng/mL组和1 μg/mL组神经干细胞形成神经球的直径、吸光度值,以及分化后的NSE、GFAP阳性细胞数与溶媒组比较,差异有统计学意义(均P<0.05)。结论Prdx6具有促进神经干细胞增殖和分化的作用。
Abstract:
Objective To investigate the effects of peroxidase 6(Prdx6) on the proliferation and differentiation of neural stem cells. Methods(1) Neural stem cells were isolated from the brain of newborn SD rats by suspension culture and passed to the third generation for immunofluorescence detection of specific protein nestin(Nestin). (2) Cell experiment group: solvent, Prdx6 1 ng/mL, 10 ng/ mL, 100 ng/mL and 1 μg/mL groups. (3) The number of cells on the first day,the second day, the third day, the fourth day, and the fifth day of each group of cells was counted by trypan blue cell counting method and a growth curve was drawn. (4) In the proliferation experiment, the diameter of the neurospheres were compared between the cells of the cell and The Cell Counting kit-8(CCK-8) was used to compare the absorbance at 450 nm of each group of cells. (5) In the cell differentiation experiment, the number of neuronal marker protein neuron specific enolase(NSE) and glial cell marker protein glial fibrillary acidic protein(GFAP) positive cells was compared by immunofluorescence staining. Results(1) In vitro cultured neural stem cell spheres were obtained. The specific marker protein Nestin was positive for immunofluorescence staining. (2) When the concentration of Prdx6 was 100 ng/mL and 1 μg/mL, the neurosphere diameter, absorbance value and the number of differentiated NSE and GFAP positive cells in the neural stem cells were statistically significant compared with the solvent group(all P<0.05). ConclusionThe proper concentration of Prdx6 can promote the proliferation and differentiation of neural stem cells.

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更新日期/Last Update: 2020-02-19